Detection of Putatively Thermophilic Anaerobic Methanotrophs in Diffuse Hydrothermal Vent Fluids
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چکیده
منابع مشابه
Detection of putatively thermophilic anaerobic methanotrophs ( ANMEs ) in 1 diffuse hydrothermal vent fluids 2 3
3 Alexander Y. Merkel#, Julie A. Huber , Nikolay A. Chernyh, 4 Elizaveta A. Bonch-Osmolovskaya, Alexander V. Lebedinsky 5 6 Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-Letiya 7 Oktyabrya 7/2, 117312 Moscow, Russia, and Josephine Bay Paul Center, Marine Biological 8 Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA 9 10 Running title: Thermophilic ANMEs in d...
متن کاملDetection of putatively thermophilic anaerobic methanotrophs in diffuse hydrothermal vent fluids.
The anaerobic oxidation of methane (AOM) is carried out by a globally distributed group of uncultivated Euryarchaeota, the anaerobic methanotrophic arachaea (ANME). In this work, we used G+C analysis of 16S rRNA genes to identify a putatively thermophilic ANME group and applied newly designed primers to study its distribution in low-temperature diffuse vent fluids from deep-sea hydrothermal ven...
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Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vent...
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Published pmoA primers do not match the pmoA sequence of "Candidatus Methylomirabilis oxyfera," a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of "Ca. Methylomirabilis oxyfera"-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular too...
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At deep-sea hydrothermal vents chemolithoautotrophic microbes mediate the transfer of geothermal chemical energy to higher trophic levels. To better understand these underlying processes and the organisms catalyzing them, this research used DNA Stable Isotope Probing (SIP) combined with Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) to identify the microo...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2013
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03034-12